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関連する概念動画

Gene Conversion02:08

Gene Conversion

9.6K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.6K
Homologous Recombination02:31

Homologous Recombination

50.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.1K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Exon Recombination02:32

Exon Recombination

3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.5K
Crossing Over01:30

Crossing Over

4.1K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.1K
CRISPR01:59

CRISPR

49.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: May 30, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

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リピート要素の再組み合わせによるヒトゲノムのランダム化

Jonas Koeppel1, Raphael Ferreira2,3,4, Thomas Vanderstichele1

  • 1Wellcome Sanger Institute, Hinxton, UK.

Science (New York, N.Y.)
|January 30, 2025
PubMed
まとめ
この要約は機械生成です。

研究者はCRISPRプライムエディティングを用いた ゲノムスクランブル戦略を開発し,非コーディングDNAを研究した. この方法は大きなDNAの再編成を生み出し,ゲノム組織が遺伝子発現と選択圧力にどのように影響するか明らかにします.

さらに関連する動画

Recombineering Homologous Recombination Constructs in Drosophila
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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

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関連する実験動画

Last Updated: May 30, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

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Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering Homologous Recombination Constructs in Drosophila

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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Published on: November 20, 2016

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科学分野:

  • ゲノミクスと分子生物学
  • エピジェネティクスと遺伝子調節

背景:

  • 現在のツールは大規模DNA編集には不十分で,99%のコード化されていないヒトゲノムの研究を妨げています.
  • 非コーディングDNAの理解は ゲノム組織と遺伝子調節を解読するのに不可欠です

研究 の 目的:

  • ノンコーディングゲノムにおける大規模なDNA操作のための新しいツールを開発する.
  • 誘導された再編成を通じてゲノム欠かせないことと組織的原理を調査する.

主な方法:

  • リコンビネーションハンドルを 繰り返しのシーケンスに挿入するために CRISPR プライムエディティングを適用する.
  • リコンビネーズの誘導により,ストキャスティックメガベースサイズのDNAの再編成 (削除,逆転,転位,円形のDNA) が発生する.
  • 選択圧力を評価し,結果のクローンを特徴付けるために,時間とともに再配置を追跡します.

主要な成果:

  • 細胞ごとに 100 メガベース規模の 再編成を成功させた
  • 重要な遺伝子を回避するより短い変種を好む観察された選択
  • 変異体内の遺伝子の発現に影響を及ぼすが,隣接する遺伝子は影響しないことを示す特徴化されたクローン.

結論:

  • 開発されたゲノムスクランブル戦略により 前例のない大規模なDNA編集が可能になります
  • このアプローチは,ゲノム組織,必要性,遺伝子発現に対する構造的変異の影響を研究することを容易にする.