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関連する概念動画

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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Membrane Fluidity01:23

Membrane Fluidity

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Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
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Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH01:21

Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH

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Drug absorption within the gastrointestinal (GI) tract is a complex process influenced by several critical factors, including the site pH, the drug's dissociation constant (pKa), and the drug's lipophilicity. The GI tract exhibits a pH gradient, with an acidic environment in the stomach and a more alkaline environment in the small intestine. This pH variation directly affects the ionization state of drugs.
A drug's pKa and the pH of the gastrointestinal (GI) tract play crucial roles...
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Membrane Lipids01:32

Membrane Lipids

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Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
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Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

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Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
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  1. ホーム
  3. 構造とph 断片化された酸化フォスフォリピドによる膜分解機構の依存性
  1. ホーム
  3. 構造とph 断片化された酸化フォスフォリピドによる膜分解機構の依存性

関連する実験動画

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

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構造とpH 断片化された酸化フォスフォリピドによる膜分解機構の依存性

Min Xie1, Maik G N Derks1,2, Eveline H W Koch1

  • 1Membrane Biochemistry & Biophysics, Bijvoet Center for Biomolecular Research, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Journal of the American Chemical Society
|March 5, 2025

PubMed で要約を見る

まとめ
この要約は機械生成です。

酸化した断片化フォスフォリピドは細胞膜を破壊し,分子構造とpHによって影響が異なる. これらの発見は,pH反応性薬剤投与システムの開発に役立つかもしれない.

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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

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関連する実験動画

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

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科学分野:

  • 生物化学
  • 膜生物物理学
  • 酸化ストレスに関する研究

背景:

  • 膜脂質の酸化は酸化ストレスにおける重要なプロセスであり,細胞死につながる酸化縮小型リンパ脂質を生成する.
  • これらの酸化した脂質が膜の性質を変化させる正確なメカニズムとpHのような環境要因の影響は十分に理解されていません.

研究 の 目的:

  • 切断されたアシル鎖を持つ個々の脂質アルデヒドとカルボキシル酸が,モデル膜の構造と機能にどのように影響するかを調査する.
  • 酸化した断片化された脂質が膜の浸透性と曲線に及ぼす影響を調節するpHの役割を解明する.

主な方法:

  • 特定の切断された脂質アルデヒドとカルボキシル酸 (ΔC9とΔC5) の影響を研究するためにモデル膜システムを使用した.
  • 異なる電荷と大きさの分子に対する膜浸透性を評価した.
  • 膜の曲線と脂質の振る舞いのpH依存の変化を分析した.

主要な成果:

  • 脂質アルデヒドとカルボキシル酸は,ΔC9が一般的にΔC5よりも有効であるとの差異的な膜浸透効率を示す.
  • ΔC9の縮小した脂質カルボキシル酸は,カルボキシル群イオン化に関連して,pH依存の膜曲線と浸透性を誘導する.
  • 切断された脂質はインターフェースに移動し,潜在的な細胞間シグナル伝達を示唆します.
  • アルデヒドと非イオン化カルボキシルは,曲線を誘導することなく,より大きな分子への透過性を高めます.

    • 結論:

    • 断片化されたアシル鎖を持つ酸化されたリン酸は,その特定の分子特性と環境pHに依存する方法で膜構造を破壊する.
    • 酸化した脂質のpHに依存する行動は,pH反応性脂質ナノ粒子などの標的型薬物投与システムを設計する可能性を秘めています.