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Updated: May 24, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

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テロメアトランポゾンは,線形細菌のゲノムに浸透している

Shan-Chi Hsieh1, Máté Fülöp2, Richard Schargel1

  • 1Department of Microbiology, Cornell University, Ithaca, NY, USA.

Science (New York, N.Y.)
|March 6, 2025
PubMed
まとめ

サイアノバクテリアとストレプトマイケスのテロメアトランポゾンでは,線形ゲノムを維持するために独立して進化することができる. ゲノムの進化と 遺伝子の移転を促し 移動性要素は 原生テロメアを 置き換えることができます

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DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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LTR Retrotransposons

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The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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There are several different mechanisms used to attenuate transcription. In ribosome mediated...
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