Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

12.1K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
12.1K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.4K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.4K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

76.9K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
76.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Optimizing phage therapy with antidefense proteins acquired from the environment.

Frontiers in microbiology·2026
Same author

Intermolecular C─H···M (M = Fe and V) Interactions in Metalloporphyrins: A Combined Experimental and Computational Perspective.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Zinc-Porphyrin Terephthalate-Based Metal-Organic Framework: Structural Insights and Functional Antibacterial Properties.

Chembiochem : a European journal of chemical biology·2026
Same author

Diversity of DNA viruses in the atmosphere of sub-Antarctic South Georgia.

Frontiers in microbiology·2026
Same author

Incorporation of Functional Proteins on Cellular Surfaces via Artificial Cell-Derived Vesicles (ACDVs) for Plasma Membrane Reprogramming.

Journal of the American Chemical Society·2026
Same author

Polymeric Lysosome-Targeting Chimeras (PolyTACs): Extracellular Targeted Protein Degradation without Co-Opting Lysosome-Targeting Receptors.

Journal of the American Chemical Society·2026

関連する実験動画

Updated: May 23, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.2K

デュアルアクションのみの PROTAC

Ranit Dutta1,2, Anirudh Devarajan1,2, Amelia Talluri1

  • 1Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.

Journal of the American Chemical Society
|March 10, 2025
PubMed
まとめ
この要約は機械生成です。

この研究では,新薬技術であるダブル・アクション・オンリー PROTACs (DAO-PROTACs) が導入されています. これらのPROTACの活性化には,低酸素とキャセプシン-Lの両方が必要であり,副作用を軽減し,安全性を改善します.

さらに関連する動画

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
05:33

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

9.4K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K

関連する実験動画

Last Updated: May 23, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

3.2K
High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
05:33

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

9.4K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K

科学分野:

  • 薬の発見と開発
  • 分子生物学
  • 生物化学

背景:

  • タンパク質分解を標的とするキメラ (PROTACs) は強力な偽触媒薬である.
  • PROTACをターゲットにすると 望ましくない結果が生じます
  • 標的型タンパク質分解は 有望な治療戦略です

研究 の 目的:

  • 標的型タンパク質分解のための一般化可能なAND-ロジックゲートされたPROTACシステムを開発する.
  • 二重疾患に関連する内生刺激によって活性化されるPROTAC分子を設計する.
  • PROTACベースの治療法に関連した細胞毒性を軽減するために.

主な方法:

  • 二重作用のみのPROTAC (DAO-PROTAC) 分子の設計と合成
  • 低酸素とキャセプシンLを必要とする二重刺激の活性化メカニズムの開発
  • DAO- PROTACの活性と細胞毒性を自由PROTACと単発PROTACと比較した評価

主要な成果:

  • DAO- PROTACsは,低酸素とキャセプシン- Lの同時存在によって活性化されるまで休眠状態です.
  • DAO- PROTACsの活性化は,関心のあるタンパク質 (POI) の分解につながります.
  • DAO- PROTACは従来のPROTACと比較して細胞毒性が著しく低下しています.

結論:

  • DAO-PROTACは,精密なタンパク質分解のための新しいAND-ロジックゲートされたアプローチを表しています.
  • 二重刺激の活性化により,PROTACベースの治療法の安全性が向上します.
  • この技術はより安全で効果的な 標的型タンパク質分解治療の可能性を秘めています