(+) -および (-) -オーランチン のエナンチオセレクティブバイオシンセシス
Shinji Kishimoto1, Tsubasa Tamura1, Takumi Okamoto1
1Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Journal of the American Chemical Society
|March 18, 2025
まとめ
アスペルギルス・レントゥルス菌株は,別々の生合成経路を通じて,天然の製品であるオーランチン (auranthine) のエナントイオマーを産生する. この研究は,ニトリルを含むアミノ酸の生物合成と真菌のエナチオメリック天然産物の生産を明らかにしています.
科学分野:
- * 菌類と自然産物の化学
- * 生化学と分子生物学
背景:
- * 生物活性天然産物 (NP) は,生物合成中に通常立体化学的に制御されます.
- * 酵素のステレオ特異性により,真菌代謝産物の中でエナンチオマー (鏡像同位体) は稀に見られる.
- オーランチンはニトリル基を含む真菌NPであり,その生物合成は以前は特徴づけられていなかった.
研究 の 目的:
- * 異なるアスペルギルス・レントゥルス菌株によって産生されるオーランチンエナティオメアの生物合成を調査する.
- * (-) - と (+) - アウランチン同位体を生成する遺伝子と酵素を特定する.
- * 菌類におけるニトリル含有アミノ酸の生物合成の仕組みを解明する.
主な方法:
- * 関連する遺伝子を特定するためのトランスクリプトミックの分析
- * 遺伝子の機能を確認するための遺伝子消去と異質発現実験
- * 酵素活性を示すための生化学的測定
主要な成果:
- 2つの異なる非リボソームペプチド合成酵素 (NRPSs),NitAとNitCを特定し,それぞれ (-) - と (+) - アウランチンを合成する.
- *NitCはエピメリゼーションドメインを有し,エナティオメアペアの生成を説明した.
- ニトリルを含むアミノ酸l-γ-シアノホモアラニンの生物合成を触媒するアルギニノスッキナート合成酵素であるNitBの特徴.
結論:
- * 異なるアスペルギルス・レントゥルス菌株は,異なるNRPS酵素 (NitAとNitC) を介してオーランチンのエナントオマーを生成する.
- * NitC のエピメリゼーションドメインの存在は,エナティオメアペアの生成の鍵です.
- * ニトリルを含むアミノ酸の生合成のための新しい経路が明らかにされました.
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