平面細胞の極性複合体による上皮質の極化は,独占的に非細胞自律である
Lena P Basta1, Bradley W Joyce1, Eszter Posfai1
1Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
まとめ
細胞極化には,平面細胞極性 (PCP) 複合分離のための細胞-細胞接触が必要です. PCP複合体の細胞間結合は非対称性を引き起こし,細胞自体の相互作用を引き起こさない.
科学分野:
- 細胞生物学
- 発達生物学
- 組織の極性
背景:
- 組織平面に沿った集団的な細胞の極化は,非対称な平面細胞極性 (PCP) 複合体によって細胞間接触を形成する.
- 非対称なPCP複合体の分離のために細胞との接触の必要性は不明である.
- 分極化のために細胞自律的な相互作用が十分であるかどうかは不明である.
研究 の 目的:
- 平面細胞極性 (PCP) 複合体の非対称分離が細胞と細胞の接触を必要とするかどうかを調査する.
- 細胞自律的,敵対的な相互作用が二極化に十分かどうかを決定する.
主な方法:
- 二重PCPレポーター細胞とPCPブリッジを形成できないラベルのない細胞を持つマウスキメラの生成.
- 細胞間相互作用がない場合の PCP タンパク質の行動の観察
- 単細胞と単細胞のインターフェースでのPCPコンポーネントの分類の分析.
主要な成果:
- 平面細胞極性 (PCP) タンパク質は,細胞間相互作用なしに細胞自律的に極化することができなかった.
- 単細胞インターフェイスでのPCP媒介接触は,PCP成分を交差点の反対側に分離するのに十分でした.
- この分類は,交差点の方向性とは無関係に発生した.
結論:
- 平面細胞極性 (PCP) 複合体の細胞間結合は,非対称性の開始に不可欠である.
- PCP複合体の分離と分極化の決定的なステップは,細胞間相互作用ではなく,細胞間接触である.
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