関連する実験動画

Updated: Jun 15, 2025

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
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DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

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変異したミトコンドリアDNAポリメラーゼの活性を再生する小分子

Sebastian Valenzuela1, Xuefeng Zhu1, Bertil Macao1

  • 1Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.

Nature
|April 9, 2025
PubMed
まとめ

科学者はミトコンドリア DNA (mtDNA) の合成を活性化する新しい小分子 PZL-A を発見した. この化合物は変異したDNAポリメラーゼガンマ (POLγ) の機能を回復させ,POLG障害とmtDNA枯渇疾患の治療に希望を与えます.

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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

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Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
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