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関連する概念動画

Transcription Factors02:16

Transcription Factors

75.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.5K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.3K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.3K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.6K
General Transcription Factors01:30

General Transcription Factors

5.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.1K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.2K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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関連する実験動画

Updated: May 21, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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DNAによる転写因子の相互作用は,ヒトの遺伝子調節コードを拡張する.

Zhiyuan Xie1, Ilya Sokolov2,3, Maria Osmala4

  • 1State Key Laboratory of Cardiovascular Diseases and Medical Innovation Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.

Nature
|April 9, 2025
PubMed
まとめ

研究者はヒトの転写因子 (TF) 間の相互作用をマッピングし,遺伝子規制コードを理解した. 彼らは数千のTF-TF相互作用と 新しい複合モチーフを特定し 細胞の運命と発達に関する洞察を明らかにしました

さらに関連する動画

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

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関連する実験動画

Last Updated: May 21, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

8.7K
Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

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7.9K
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

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科学分野:

  • ゲノミクス
  • 分子生物学
  • システム生物学

背景:

  • DNAに結合する転写因子 (TF) は,細胞の運命と転写プログラムの決定的な遺伝子規制コードを形成する.
  • ヒトの遺伝子調節は複雑で,1,600以上の相互作用するTFが含まれていますが,DNAに結合した相互作用の風景は依然として定義が不十分です.

研究 の 目的:

  • DNAに結合したヒトの転写因子の生化学的相互作用をマッピングする.
  • TF-TF結合の好み,相互作用,結合DNA配列を同時に特定する.
  • 相互作用するTFペアによって形成された新しい複合モチーフを発見する.

主な方法:

  • CAP-SELEX (Cell-Array Processing - Exponential Enrichmentによるリガンドの体系的な進化) を利用した.これは,高通量メソッドである.
  • 相互作用するペアとDNA結合の好みを特定するために 58,000 以上の転写因子-転写因子ペアをスクリーニングしました.

主要な成果:

  • 2,198の相互作用TFペアを特定し,1,329は特定のモチーフの配列に優先的に結合している.
  • 個々のTFモチーフとは異なる1,131の新しいTF-TF複合モチーフを発見した.
  • ヒトのTF-TFモチーフの18%から47%が検出されたと推定されています.

結論:

  • 新しい複合モチーフは,細胞型特異な要素で濃縮され,in vivoで活性化され,しばしば発達的に共表現されたTFの間に形成されます.
  • 胚性軸を定義するTF間の相互作用は,類似のTFがこれらの軸に沿って異なる細胞タイプを指定する方法を説明します.