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Updated: Jun 16, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
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Oligo-LiveFISHを用いたクロマチンDNA通信の高解像度ダイナミックイメージング

Yanyu Zhu1, Ashwin Balaji2, Mengting Han1

  • 1Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.

Cell
|April 16, 2025
PubMed
まとめ

Oligo-LiveFISHは,生細胞の特定のDNA領域を前例のない解像度でリアルタイムで追跡できます. この新しい方法は 細胞の機能や病気を理解するために 重要な ダイナミックなゲノム組織を可視化します

キーワード:
CRISPRイメージングDNA通信オリゴ・ライブフィッシュダイナミック・トラッキング強化剤と促進剤の相互作用高解像度イメージングライブDNAイメージング繰り返さないゲノムイメージングポリマーモデリング主要なセル

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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