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トウモロコシの細胞型特異的な cis 調節の遺伝的構造
Alexandre P Marand1, Luguang Jiang2, Fabio Gomez-Cano2
1Department of Genetics, University of Georgia, Athens, GA, USA.
まとめ
この研究は,トウモロコシのシス調節要素の遺伝的変異が遺伝子発現と特性にどのように影響するかを明らかにしています. トランポゾン活動と局所的な適応がトウモロコシをどのように形作っているかを示しています.
科学分野:
- ゲノミクス
- 植物生物学
- 進化 的 な 遺伝子
背景:
- 遺伝子発現と複雑なフェノタイプは,シス調節要素によって調節される.
- 遺伝子変異がシス調節に及ぼす影響を理解することは 極めて重要ですが 限られています
研究 の 目的:
- 単細胞ゲノミクスを用いてトウモロコシのシス調節多様性を調査する.
- 飼育されたトウモロコシに特有のシス規制要素を特定する.
- 遺伝的変異がクロマチンのアクセシビリティと特性の変異にどのように影響するかを決定する.
主な方法:
- 172種のトウモロコシの 700万個以上の核の単細胞ゲノム解析
- 遺伝子変異を細かくマッピングするための集団遺伝学の原理
- 特徴の変異と集団の差異化による変異データの統合.
主要な成果:
- 栽培トウモロコシに特有のシス調節要素とトランポゾン活動の役割を発見した.
- 細かくマッピングされた ~22,000のクロマチンのアクセシビリティに関連した変種で,細胞タイプ特異的な効果があります.
- TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORの結合部位において,クロマチンのアクセシビリティの重要な決定因子として識別された変種.
結論:
- 局所的な適応は 特定の細胞環境で 規制ネットワークを再構築しました
- 規制ネットワークの変更は,トウモロコシの開花時間に影響します.
- シス調節要素の遺伝的変異は,トウモロコシの表型多様性の主要な原動力である.
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