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Updated: May 10, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

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ランタニドとアンテナ染色体の近接エンコーディングによる光タンパク質の設計と進化

Jinyu Wang1, Xingfeng Liu1, Kaitong Li1

  • 1Department of Chemistry, College of Science, Guangdong Provincial Key Laboratory of Catalysis, Guangming Advanced Research Institute, Southern University of Science and Technology, Shenzhen 518055, China.

Journal of the American Chemical Society
|April 23, 2025
PubMed
まとめ

長い寿命を持つ 遺伝子工学により作られた 光タンパク質は 新しい生物学的イメージングツールを提供します この研究では,高度な検出アプリケーションのために,明るさと感度が向上した新しいタンパク質を開発しました.

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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