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関連する概念動画

GPCR Desensitization01:12

GPCR Desensitization

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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

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G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
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G Protein-coupled Receptors01:15

G Protein-coupled Receptors

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G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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G-protein Coupled Receptors01:21

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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Selectins01:25

Selectins

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Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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ヒトのキモカイン-GPCR相互作用ネットワークにおける選択性および乱交性のエンコードとデコード

Andrew B Kleist1, Martyna Szpakowska2, Lindsay J Talbot3

  • 1Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA; Medical Scientist Training Program, Medical College of Wisconsin, Milwaukee, WI, USA; MRC Laboratory of Molecular Biology, Cambridge, UK.

Cell
|April 24, 2025
PubMed
まとめ

研究者は,キモカインとGタンパク質結合受容体 (GPCR) の相互作用を制御する主要な分子決定因子を特定しました. これらの要素を理解することで,治療用途のための新しいケモカインを設計することができます.

キーワード:
GPCR についてケモカイン化学反応データサイエンス機械学習多様性タンパク質とタンパク質の相互作用選択性の決定因子短い線形モチーフ構造化されていないタンパク質

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Last Updated: May 10, 2025

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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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科学分野:

  • 免疫学
  • 分子生物学
  • 構造生物学

背景:

  • ケモカイン受容体との相互作用は,46のリガンドと23のGタンパク質結合受容体 (GPCR) を含む複雑なネットワークを形成し,細胞移動に不可欠である.
  • GPCRに対するセレクティブで乱交的なケモカインの結合の分子基盤は,共通の構造構造にもかかわらず,ほとんど不明のままである.

研究 の 目的:

  • ケモカイン-GPCR相互作用における選択性と乱交性を決定する分子原理を解明する.
  • これらの特定のタンパク質とタンパク質の相互作用を媒介する保守的および可変的決定因子を特定する.

主な方法:

  • ケモカインとGPCR配列内の保存された,半保存された,および変数の決定因子の分析.
  • 構造化および非構造化タンパク質領域におけるリガンドおよび受容体によるこれらの決定因子の組み合わせ認識.
  • ウイルスのケモカインを設計し,特定された原則に基づいて変化したGPCRカップリングの好みを実証する.

主要な成果:

  • ケモカイン-GPCR結合特異性を支配する保存および変数認識要素のセットの識別.
  • 選択性と乱交性は,一般的および特定の決定因子の組み合わせから生じる.
  • 改変されたGPCR相互作用プロファイルを持つウイルスのケモカインの成功エンジニアリング.

結論:

  • ケモカイン-GPCR相互作用の特異性は,保存された分子決定因子と変数分子決定因子の組み合わせコードによって決定される.
  • これらの発見は,ケモカイン-GPCRの相互作用を理解し,設計するための枠組みを提供します.
  • この研究は,免疫療法と細胞治療の開発のためのタンパク質設計に役立つウェブリソースを提供します.