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Updated: Jun 12, 2025

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
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Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

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アリルとアルキルチオルのオートホーガン・サイト・スペシフィック・デュアル・バイオコンジュガション

Mark A R de Geus1, Christian E Stieger1,2, Jan Vincent V Arafiles1

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Straβe 10, Berlin 13125, Germany.

Journal of the American Chemical Society
|May 5, 2025
PubMed
まとめ

アリルチオールを用いて サイト固有のタンパク質を 標識する新しい生物結合法を開発しました この技術により,タンパク質の正交二重変異が可能になり,高度な抗体-薬物結合体およびナノボディが作られます.

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Preparation and Reactions of Thiols02:33

Preparation and Reactions of Thiols

Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.
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Preparation and Reactions of Sulfides02:26

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Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
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Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
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Alkynes to Aldehydes and Ketones: Hydroboration-Oxidation

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