Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Formation of Complex Ions03:45

Formation of Complex Ions

24.0K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
24.0K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.6K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.6K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

2.3K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
2.3K
Pore Transport and Ion-Pair Transport01:17

Pore Transport and Ion-Pair Transport

669
Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
669
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

683
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
683
Ion Exchange01:17

Ion Exchange

671
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
671

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

The <i>Staphylococcus aureus</i> carotenoid staphyloxanthin modifies the structure of phosphoglycerol lipid bilayers.

bioRxiv : the preprint server for biology·2026
Same author

The Interplay of Splicing and Metabolism in Cancer.

Cells·2026
Same author

Functional and structural basis of a hypermorphic TRPC3 variant.

Science advances·2026
Same author

Dynamic signature of activity-stability tradeoff in lactamase evolution.

Nature communications·2026
Same author

Alteration of the Lipid Bilayer Structure by Mg<sup>2</sup>.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Phosphates and Phosphorylated Amino Acids for the AMOEBA-HFC Polarizable Force Field.

Journal of chemical theory and computation·2025

関連する実験動画

Updated: Sep 20, 2025

Solid-state Graft Copolymer Electrolytes for Lithium Battery Applications
05:33

Solid-state Graft Copolymer Electrolytes for Lithium Battery Applications

Published on: August 12, 2013

21.8K

細胞溶液におけるATPイオン複合とリチウムの生物活性形態

Julian M Delgado1, Peter S Klein2, Sameer Varma1,3

  • 1Department of Molecular Biosciences, University of South Florida, 4202 E. Fowler Ave., Tampa FL-33620, United States.

Journal of the American Chemical Society
|May 23, 2025
PubMed
まとめ

リチウム (Li+) はATP-Mg複合体と結合し,双極性障害の治療効果に影響を与える. この研究は,Li+が細胞内で自由と結合の両方で存在し,その分子機構に影響を及ぼすことを明らかにしています.

さらに関連する動画

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

13.1K
Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques
10:03

Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques

Published on: November 11, 2013

25.6K

関連する実験動画

Last Updated: Sep 20, 2025

Solid-state Graft Copolymer Electrolytes for Lithium Battery Applications
05:33

Solid-state Graft Copolymer Electrolytes for Lithium Battery Applications

Published on: August 12, 2013

21.8K
Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature
11:04

Synthesis of Ionic Liquid Based Electrolytes, Assembly of Li-ion Batteries, and Measurements of Performance at High Temperature

Published on: December 20, 2016

13.1K
Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques
10:03

Characterization of Electrode Materials for Lithium Ion and Sodium Ion Batteries Using Synchrotron Radiation Techniques

Published on: November 11, 2013

25.6K

科学分野:

  • 生物化学
  • コンピュータ化学
  • 薬理学について

背景:

  • リチウム (Li+) は双極性障害の重要な治療法ですが,その正確な分子メカニズムはまだ不明です.
  • Li+の生物活性形態を理解することは,すべての提案された治療効果の仮説に不可欠です.
  • 自由対アデノシン三酸化物 (ATP) 結合のLi+の細胞分布は,重要な未知数である.

研究 の 目的:

  • 細胞条件下でLi+,他の単価イオン (Na+,K+),およびマグネシウム結合ATP (ATP·Mg) の結合相互作用を調査する.
  • 生理学的濃度で自由対ATP結合のLi+の分数を決定する.
  • Li + 療法に関連するイオン-ATP結合に関する構造的,熱力学的,および運動的洞察を提供する.

主な方法:

  • 分子ダイナミクス (MD) のシミュレーションは,極化可能なAMOEBA-HFC力場を使用しています.
  • 量子力学と実験データを MD力場と比較する
  • MDと実験データでパラメータ化された運動モデリング.

主要な成果:

  • ATP·Mgは,単価イオンのための2つの結合部位を有し,同時に結合することができる.
  • Li+は,これらのATP·Mg結合部位でNa+とK+と競合する.
  • Li+は高い親和性を有しているが,ATP·Mgによる細胞結合は濃度に依存し,ATPレベルの影響で生理学的極限で50%まで結合する.

結論:

  • 自由とATP結合の2つの形態のLi+は,細胞内の有意な分数で共存することができる.
  • この発見は,双極性障害の治療におけるLi+作用の分子メカニズムを探求するための基礎を提供します.
  • この研究は,細胞環境におけるイオン-ATP相互作用に関する新しい洞察を提供し,Li+の生物活性形態を特定しています.