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関連する概念動画

Enzyme Kinetics01:19

Enzyme Kinetics

99.1K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
99.1K
Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

7.8K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
7.8K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

8.2K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
8.2K
Redox Reactions01:27

Redox Reactions

221
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
221
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

20.9K
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
20.9K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.2K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.2K

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関連する実験動画

Updated: Sep 20, 2025

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

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電気駆動の酵素動的運動酸化

Beibei Zhao1, Yuanyuan Xu1, Qin Zhu1

  • 1State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Frontier Interdisciplinary Science Research Center, Nanjing University, Nanjing, China.

Nature
|May 28, 2025
PubMed
まとめ
この要約は機械生成です。

この研究は,チアミン依存酵素を再構成するためにフェロセンを用いた新しい電気酵素法を導入しています. このアプローチはアルデヒドの非自然な酸化を可能にし,高エナティオメール過剰を持つ生物活性 (S) プロフェンを生成する.

さらに関連する動画

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
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Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

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関連する実験動画

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09:58

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Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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科学分野:

  • 合成化学
  • 生物触媒
  • 電気化学

背景:

  • 酵素再利用と合成戦略は 化学的空間を広げます
  • 電気化学と酵素を統合することは,互換性や電子伝送の問題のために困難です.
  • 既存の電気酵素学的方法は,しばしば既知の酵素機能を複製する.

研究 の 目的:

  • 新しい酵素の反応性を解き放つための新しい電気酵素学的アプローチを開発する.
  • 非自然な酸化反応のために シアミン依存酵素を再構成する
  • 生物活性プロフェンを合成する

主な方法:

  • チアミン依存酵素を改変するために,フェロセンの媒介による電気触媒を用いた.
  • 改変された酵素は,α分岐アルデヒドの動的運動酸化に使用された.
  • プロセスは酵素の負荷に最適化され,全細胞でテストされました.

主要な成果:

  • 電気酵素法では,α-枝分断アルデヒドの非自然な動的動的酸化が達成された.
  • バイオアクティブ (S) プロフェンは,99%までのエナティオメール過剰で合成されました.
  • このアプローチは,全細胞と低酵素負荷 (0.05%mol) で適用できることを示した.

結論:

  • 開発された電気酵素戦略は,新しい反応性のために酵素機能を成功裏に再構築しました.
  • この方法は,エナティオメリックに純粋なプロフェンへの効率的な経路を提供します.
  • 機械学的研究により,精密な基板差別,レース化加速,効率的な電子移転が明らかになった.