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Updated: Sep 18, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

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In Vitro,セルラー,および光誘導二極EPRスペクトロスコーピーの潜在的なスピンラベルとしての小タンパク質領域

Andreas Günter1, Susanna Ciuti2, Lukas Denkhaus3

  • 1Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, 79104 Freiburg, Germany.

Journal of the American Chemical Society
|June 23, 2025
PubMed
まとめ

小さな光酸素電圧 (LOV) ドメインは,電子パラマグネティック共振 (EPR) スペクトロスコーピーの新しいタンパク質スピンラベルとして機能する. これらの遺伝的にコードされたラベルは,光活性化フラビンモノヌクレオチド (FMN) のラジカルを使用して,タンパク質と細胞内の距離測定を可能にします.

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