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Updated: Sep 17, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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酵素酸化結合によるチロールタグ付きタンパク質への反応性チオルハンドルの導入

Paul Huang1, Wendy Cao1, Jennifer L Fetzer1

  • 1Department of Chemistry, University of California, Berkeley, California 94720, United States.

Journal of the American Chemical Society
|June 27, 2025
PubMed
まとめ

新しいチロシナーゼ酵素変種であるメガTYRは,チロシン残基をディチオトリトール (DTT) を使用してチオールハンドルに変換することによって,効率的なタンパク質結合を可能にします. これは抗体と薬物の結合物および他の生物結合治療薬の生成を容易にする.

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