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関連する概念動画

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

220
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
220
CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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関連する実験動画

Updated: Sep 11, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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フォト制御可能なプログラム可能な酵素カスケード 堅牢なCRISPR診断

Menglu Hu1, Yihui Wang1, Weiwei Qi1

  • 1School of Life Sciences, South China Normal University, Guangzhou 510631, China.

Journal of the American Chemical Society
|August 13, 2025
PubMed
まとめ

この研究は,核酸検出のための新しい光制御CRISPR診断システムを導入します. この技術は,プロトスペーサーの隣接モチーフの制限を克服し,同時に二重遺伝子検出を可能にし,診断能力を向上させます.

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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関連する実験動画

Last Updated: Sep 11, 2025

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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科学分野:

  • バイオテクノロジー
  • 分子診断
  • CRISPR技術について

背景:

  • CRISPR-Cas12aの診断は高度な核酸検出が可能ですが 限界があります
  • プロトスペーサーの隣接モチーフ (PAM) の要件はターゲットサイトの選択を制限します.
  • マルチプレキシング機能の制限により,複数の標的を同時に検出することが困難です.

研究 の 目的:

  • CRISPR診断システムを開発しました
  • PAMの制約を克服し,CRISPR診断におけるマルチプレクスを強化する.
  • 標的遺伝子の同時検出と内部の制御を可能にし,臨床有用性を向上させる.

主な方法:

  • 光制御された酵素カスケード戦略が採用された.
  • 配列反応には,核酸増強,ラムダ外核酶によるssDNA生成,およびPAM独立のCas12a検出が含まれていた.
  • Cas12aとCas13aのオートゴーナル・トランス・クリバージは,二重遺伝子の検出を容易にした.

主要な成果:

  • このシステムは,PAMから独立した検出を成功させた.
  • Cas12aとCas13aを用いて同時に二重遺伝子の検出が実証された.
  • Mycobacterium tuberculosis (MTB) の臨床サンプルと内部制御遺伝子 (ACTB) が正確に検出されました.

結論:

  • CRISPRの診断技術は 柔軟性を高め 従来の方法の限界を克服します
  • このアプローチは,標的と対照遺伝子の同時に検出を可能にすることで,CRISPRベースの診断の臨床応用を進めています.
  • 開発されたシステムは,改善された分子診断のための大きな希望を示しています.