関連する実験動画

Updated: Sep 10, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

PRMT5は,Ganoderma lucidumにおけるヒストンのメチル化によるセルラーゼ遺伝子の発現を調節することによってセルラーゼの産生を促進する

Yefan Li¹, Zi Wang¹, Zhouyu Li¹

¹Key Laboratory of Agricultural Environmental Microbiology, Microbiology Department, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, People's Republic of China.

Microbial cell factories

|August 20, 2025

まとめ

タンパク質アルギニンメチルトランスフェラーゼ5 (PRMT5) は,Ganoderma lucidumのセルラーゼ生成を促進する. PRMT5はヒストンのメチル化によってセルラーゼ遺伝子EG2の発現を調節し,産業や環境への影響を与える.

科学分野:

生物化学
分子生物学
菌類学

背景:

セルロース酵素はセルロースの分解に不可欠であり,天然の炭素循環,産業プロセス,バイオエネルギーに影響を与えます.
Ganoderma lucidumは白色腐敗菌で,セルラーゼを大量に生産する菌である.
翻訳後の改変はセルラーゼの産生に影響することが知られている.

研究の目的:

Ganoderma lucidumのセルラーゼ生成の調節におけるタンパク質アルギニンメチルトランスファーゼ5 (PRMT5) の役割を調査する.
PRMT5がセルラーゼ合成に影響を与えるメカニズムを解明する.

主な方法:

ゲノムシューティングゲノムシューティングゲノムシューティング
セルラーゼの活性と菌糸体の成長率の測定
農業廃棄物の分解の分析
PRMT5結合とヒストンのメチル化を評価するために,定量PCR (ChIP-qPCR) が続くクロマチン免疫降水.
エンドグルカナーゼ2 (eg2) サイレンシングと過剰発現の株の構築と分析

主要な成果:

prmt5を静止すると,セルラーゼの活性が23%,菌糸体の成長が71%減少した.
PRMT5のノックダウンは,トウモロコシのストローとトウモロコシのコップの分解をそれぞれ約60%と51%減少させた.
CHIP- qPCRは,PRMT5がヒストンのメチル化によってエンドグルカナーゼ2 (eg2) の発現を正に調節することを示した.
EG2の過剰発現はセルラーゼの活性を増大させ,その経路における役割を確認した.

結論:

PRMT5は,Ganoderma lucidumにおけるセルラーゼの産生を正常に制御する役割を果たしています.
このメカニズムはPRMT5媒介ヒストンのメチル化により,セルラーゼ遺伝子EG2の発現を調節する.

キーワード:

セルラーゼ EG2 についてメチル化 PRMT5 について

さらに関連する動画

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Published on: June 13, 2014

関連する概念動画

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...