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Updated: Sep 8, 2025

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

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2つの成分であるフラビン依存型トリプトファンハロゲナーズのフラビン還元酵素子単位であるBorFのX線結晶構造

Zheng Ma1, Emily W Rady1, Aravinda J de Silva1

  • 1Department of Chemistry and Biochemistry, College of Natural Sciences and Mathematics, The University of Toledo, 2801 W. Bancroft St, Toledo, OH, 43606, U.S.A.

bioRxiv : the preprint server for biology
|August 20, 2025
PubMed
まとめ

フラビン還元酵素BorFは,ボリゴミシンA生物合成におけるトリプトファンハロゲネーゼBorHに不可欠なFADを減らすためにNADHを使用する. 構造分析によると,FADはBORFの減少のためにBORHから完全に分離する必要があります.

キーワード:
フラビンアデニンジヌクレオチド生物合成クリスタルグラフィーオキシドレダクトーゼ

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