Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.5K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.5K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.3K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.3K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

2D Multimodal Image Collection for Fluorescence Prediction from Transmitted Light Microscopy.

Scientific data·2026
Same author

Statistical models to characterize colon tumor stiffness heterogeneity through representative atomic force microscopy maps.

Scientific reports·2026
Same author

3D bioprinted breast cancer model reveals stroma-mediated modulation of extracellular matrix and radiosensitivity.

Bioactive materials·2024
Same author

Quantifying surface tension and viscosity in biomolecular condensates by FRAP-ID.

Biophysical journal·2024
Same author

N-Formylation modifies membrane damage associated with PSMα3 interfacial fibrillation.

Nanoscale horizons·2024
Same author

Free-Standing DNA Origami Superlattice to Facilitate Cryo-EM Visualization of Membrane Vesicles.

Journal of the American Chemical Society·2024

関連する実験動画

Updated: Sep 10, 2025

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

13.3K

相対AFM/光顕微鏡を用いた核封筒のイメージング

Emilie Costes1, Anthony Vial2, Christine Doucet3

  • 1Centre de Biologie Structurale, INSERM, CNRS, University of Montpellier, Montpellier, France.

Methods in molecular biology (Clifton, N.J.)
|August 20, 2025
PubMed
まとめ

この研究は,核孔複合体 (NPC) の構造を保ちながら,哺乳類の細胞から核封筒 (NEs) を作る新しい方法を示している. この技術は,原子力顕微鏡 (AFM) を使用して,内外核膜の高解像度イメージングを可能にします.

キーワード:
原子力顕微鏡 (AFM)直接ストキャスティック光学再構築顕微鏡 (dSTORM)光顕微鏡哺乳類の細胞核封筒核孔複合体 (NPC)単分子局所化顕微鏡 (SMLM)

さらに関連する動画

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.2K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.4K

関連する実験動画

Last Updated: Sep 10, 2025

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

13.3K
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.2K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.4K

科学分野:

  • 細胞生物学
  • バイオ物理学
  • 顕微鏡検査

背景:

  • 核膜 (NE) は,内外膜を持つ重要な真核構造である.
  • 既存のNE製剤は,膜張力喪失により核毛孔複合体 (NPC) の形状を変えることができる.
  • 原子力顕微鏡 (AFM) は,NE構造をイメージするための強力なツールです.

研究 の 目的:

  • 培養された哺乳類の細胞から無傷な核封筒 (NEs) を準備するための新しい方法を開発する.
  • 試料の準備中に核孔複合体 (NPC) の原生構造を保存する.
  • AFMと相関顕微鏡を使用して,内外核膜の両方の高解像度イメージングを可能にします.

主な方法:

  • 培養された哺乳類の細胞から核を抽出し,ガラス基板に貼り付けます.
  • 膜の緊張とNPCの整合性を維持するために,核の穏やかな開口.
  • 免疫ラベリングはAFMと直接のストキャスティック光学再構築顕微鏡 (dSTORM) と組み合わせて,相関イメージングを行う.

主要な成果:

  • この新製法では,NE膜の張力とNPC構造をうまく保ちます.
  • 内外核膜の両方の高解像度AFMイメージングが達成されます.
  • 関連AFM/dSTORMイメージングは,詳細な構造と分子情報を提供します.

結論:

  • このプロトコルは,形状が保存されたNEとNPCを研究するための堅固なアプローチを提供します.
  • この方法は,植物細胞や組織を含む様々な細胞モデルに適応できます.
  • 生物学的な洞察を深めるための 高度な相関イメージング技術です