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Updated: Sep 10, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
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Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

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核膜の核孔複合組成を研究するために,核ポリン結合ビーズの使用

Şükriye Bilir1,2, Yasushi Hiraoka1, Tokuko Haraguchi3

  • 1Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.

Methods in molecular biology (Clifton, N.J.)
|August 20, 2025
PubMed
まとめ

細胞分裂中の核毛孔複合体 (NPC) の組成を研究するための新しい方法を開発した. この技術は,ミトーシス後の核包膜の再構築における個々の核孔タンパク質 (NuPs) の機能を明らかにするのに役立ちます.

キーワード:
抗GFP抗体と結合されたビーズライブ CLEM イメージング生細胞画像検査核孔複合体ニュクレオポリンノープ・ビーズ

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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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