マルチオミクスは,トマトの長い非コーディングRNAの遺伝子調節と機能を明らかにする

Yaoyao Wu^1,2, Keyi Ye¹, Jian Yin^3,4

¹National Key Laboratory of Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China.

Plant physiology

|August 21, 2025

まとめ

トマトの繁殖中に長い非コーディングRNA (lncRNAs) が著しく変化し,果物の味に影響した. この研究では lncRNAs が

科学分野:

植物分子生物学
ゲノミクス
メタボロミクス

背景:

長い非コーディングRNA (lncRNAs) は,植物の発達と信号伝達に不可欠である.
トマト (Solanum lycopersicum) の育種中のlncRNA発現の全体的な変化と,果物の代謝産物蓄積におけるその役割は十分に理解されていません.

研究の目的:

トマトのアクセションにおける lncRNA を特定し,特徴づけること.
lncRNA発現に対する家畜化と繁殖の影響を調査する.
lncRNAsとフルーツの代謝物質の含有量と味の関連性を調査する.

主な方法:

lncRNAを特定するために,404個のトマト結合のRNA配列を解析する.
繁殖中の lncRNA発現変化と核酸多様性の分析.
lncRNA発現の定量特性の位置 (eQTL) の特定
lncRNAと果物の代謝産物プロファイルとの関連分析
lncRNAの機能を検証するトランスジェニック実験

主要な成果:

15,548 の lncRNA が特定され,2,115 が高信頼性パネルで発現した lncRNA として指定されました.
選択的繁殖に関連した776の差異的に発現するlncRNAが特定されました.
トマトの果物のメタボリート含有量と関連していた.
特定のlncRNA (lncRNA-PEL8) は,2-フェニルエタノール含有量を正に調節することが確認された.
lncRNA発現レベルはメタボリート含有量を正確に予測した.

結論:

lncRNAは,トマトの果物の全身代謝過程を調節する上で重要な役割を果たします.
lncRNA発現パターンはトマトの繁殖歴を反映している.
lncRNAはトマトの風味を高める可能性と育種用途を提供しています

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