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関連する概念動画

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

18.1K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
18.1K
Protein Folding01:25

Protein Folding

8.6K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
8.6K
Conserved Binding Sites01:49

Conserved Binding Sites

4.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.9K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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関連する実験動画

Updated: Sep 10, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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SuFEx-Enabledポリペプチド:最小限のプリセット条件で多様化された性質を作成する

Ying Liu1,2, Yajie Chen1, Xue-Ao Mei1

  • 1State Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan Province, 410082, China.

Angewandte Chemie (International ed. in English)
|August 21, 2025
PubMed
まとめ

研究者は,硫黄 (VI) -フッ素交換 (SuFEx) 化学を用いてポリペプチドを簡単に合成し,機能化するための新しい方法を開発した. この画期的な発見により 抗ウイルス作用を含む 強化された特性と潜在的な応用を持つ 新しいバイオマテリアルの設計が可能になりました

キーワード:
抗ウイルスポリマーN-カルボシアンヒドリドポリマー機能化ポリペプチドSuFEx について

さらに関連する動画

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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関連する実験動画

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

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科学分野:

  • ポリマー化学
  • バイオマテリアル科学
  • 有機合成

背景:

  • ポリペプチドの合成と機能化は,その性質と応用の探求を制限する重大な課題を提示する.
  • 既存の方法はしばしば複雑な機能化前のステップまたは反応性アンカーを必要とします.

研究 の 目的:

  • ポリペプチド合成と機能化のための簡単で効果的な方法を開発する.
  • 硫黄 (VI) フッ化物交換 (SuFEx) 分子を多機能化のためにポリペプチドに組み込む.
  • バイオマテリアルの応用のためのポリペプチドのSuFEx由来結合の性質を調査する.

主な方法:

  • アミノ酸のN-カルボシアンヒドリドにSuFEx分子を組み込む.
  • 機能化されたポリペプチドを作るためのリング開封ポリメリゼーション.
  • 硫酸塩またはアミドスルフォナート (硫酸塩) 結合によるポリペプチドの改変
  • 機能的部分との結合特性とシナージ効果の評価

主要な成果:

  • 合成したポリペプチドにSuFExの分子が組み込まれている.
  • 既定条件なしにヒドロキシルおよびアミノ基を用いたポリペプチドの容易な機能化が実証された.
  • SuFEx由来の硫酸塩と硫酸塩結合の特徴的な特性
  • 抗ウイルス活性と生物互換性を示すサッカリド改変型ポリペプチドとの概念証明でシナージ効果を示した.

結論:

  • SuFEx化学は,ポリペプチド合成と機能化のための多用途のプラットフォームを提供します.
  • SuFExから派生した結合は,新しいバイオマテリアルの設計に有益なユニークな特性を提供します.
  • SuFEx結合のサッカリド改変型ポリペプチドは,有望な抗ウイルス活性と生物相容性を示しています.