関連する実験動画

Updated: Sep 10, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Prkciは,腫瘍血管新生を促進するためにJak2/Stat3シグナルを活性化します. 略称:腫瘍血管新生におけるPrkci

Peng Li¹, Guangshi Liu¹, Wenbin Zhang²

¹Gastrointestinal Surgery department, People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang, Urumqi 830000, China.

Neoplasia (New York, N.Y.)

|August 21, 2025

まとめ

タンパク質キナーゼCIOTA (Prkci) は,Jak2/Stat3経路を活性化することで,結腸直腸がん (CRC) の血管新生を誘導する. Prkciを阻害することは,CRCの進行と腫瘍の成長と戦うための潜在的な治療戦略を提供します.

科学分野:

腫瘍学
分子生物学
癌研究

背景:

腫瘍血管新生は結腸直腸がん (CRC) の成長と転移に不可欠です.
タンパク質キナーゼCIOTA (Prkci) は腫瘍性作用があるが,CRC血管新生におけるその機能は不明である.

研究の目的:

CRCの血管新生におけるPrkciの役割を調査する.
Jak2/Stat3信号経路の関与を明らかにする.

主な方法:

CRC組織におけるPrkci発現を分析し,マイクロ血管密度と予後と相関した.
インビトロ内皮細胞検査 (増殖,移動,チューブ形成)
PrkciのノックアウトCRC異種移植マウスモデルとJak2/Stat3/Vegfaシグナル伝達に関するメカニズム研究を使用した.

主要な成果:

CRCの組織におけるPRKCIの上昇は,予後不良と血管新生の増加と相関する.
Prkciは内皮細胞機能を in vitroで強化し,ノックアウトはこれを抑制した.
PrkciはJak2 (S633) を酸化し,Stat3を活性化し,Vegfaを増加させ,血管新生を促進する.
Prkci ノックアウトは腫瘍の成長を減少させ,生存率を改善しました.

結論:

Prkciは,Jak2/Stat3信号伝達によるCRC血管新生の重要なレギュラーである.
PRKCIをターゲットにすることは,CRCに対する新しい治療戦略です.

キーワード:

血管新生大腸がん Jak2/Stat3経路についてパクチー治療目的ベジファ

さらに関連する動画

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Published on: June 23, 2023

関連する概念動画

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...