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関連する概念動画

Production Efficiency01:01

Production Efficiency

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Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
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Free-body Diagram01:28

Free-body Diagram

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In mechanics, understanding the motion of objects is essential, and one tool that helps solve this problem is the free-body diagram. It is a simple but powerful graphical representation that succinctly represents all the forces acting on an object. A free-body diagram can represent a stationary or moving object, and is used in mechanics to explain the cause of an object's motion.
A free-body diagram transforms a complex problem into a simple representation, making it easy to understand the...
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Mechanical Efficiency of Real Machines01:14

Mechanical Efficiency of Real Machines

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The mechanical efficiency of a machine is a fundamental concept that describes how effectively a machine can convert input work into output work. According to this concept, the efficiency of a machine is equal to the ratio of the output work to the input work. An ideal machine, meaning a machine that has no energy losses, has an efficiency of one. This implies that the input work and the output work are equal.
However, in reality, no machine can be truly ideal, and all of them experience some...
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Machines: Problem Solving II01:30

Machines: Problem Solving II

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Machines are complex structures consisting of movable, pin-connected multi-force members that work together to transmit forces. Consider a lifting tong carrying a 100 kg load. It comprises movable sections DAF and CBG linked together with member AB.
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Composite Bodies00:55

Composite Bodies

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A composite body is a body made up of multiple parts, connected to form a larger, unified object. Each part has its own weight and center of gravity, which must be considered to determine the center of gravity of the composite body. In cases where the density or specific weight is constant, the center of gravity coincides with the centroid.
Composite bodies have widespread applications in mechanical engineering, from automobiles to aircraft to rockets. For example, an automobile wheel comprises...
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Production of Formed Elements01:34

Production of Formed Elements

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Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
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関連する実験動画

Updated: May 2, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

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効率的なターペノイド生産のためのエンジニアリングストレプトミセス

Xiaoxu Lin1, Xingwang Xu1, Dongxu Zhang1

  • 1State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.

Journal of agricultural and food chemistry
|August 22, 2025
PubMed
まとめ

ストレプトマイケスの宿主は,従来の宿主の限界を克服して,スケーラブルなテルペノイド生産のために設計されました. この研究は,最適化された代謝経路を通じて,酸化誘導体を含む4つの複雑なテルペノイドの高収量を達成した.

キーワード:
ストレプトマイセスのシャーシエンジニアリングメチレリトールリン酸経路過剰生産テルペノイド

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
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関連する実験動画

Last Updated: May 2, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

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科学分野:

  • メタボリック・エンジニアリング
  • 合成生物学
  • 自然製品の生物合成

背景:

  • テルペノイドは農業と治療に大きな可能性を秘めているが,天然の豊富さや複雑な酸化調整により生産上の課題に直面している.
  • 従来の微生物宿主には,複雑なテルペノイド誘導体の効率的な生成に必要な酵素機構,特にP450細胞染色体がないことが多い.

研究 の 目的:

  • 構造的に複雑なテルペノイドのスケーラブルな生産のための代替微生物のシャーシを開発する.
  • ステルプトミセスの生物合成能力を活用して テルペノイド合成と酸化調整を 強化する.

主な方法:

  • 代替生産機として2つのStreptomycesホストを設計した.
  • 速度を制限するMEP経路の遺伝子を持つTriMEPカセットを特定し,組み立てるためにEcoMine戦略を使用しました.
  • 培養媒体を最適化し,強い構成プロモーターを遺伝子発現に利用した.

主要な成果:

  • シェイクボトル培養で3つの酸化製品を含む4つのテルペノイドの高レベルの生産を達成した.
  • 報告された収量には (-) -epi-α-bisabolol (237 ± 14 mg/L),ent-(13Z) -isocupressic acid (173 ± 14 mg/L),ent-(13E) -isocupressic acid (209 ± 20 mg/L),およびent-atiserenoic acid (325 ± 17 mg/L) が含まれている.
  • エンジニアリングされたStreptomycesの宿主体内で,P450媒介による酸化調整が成功していることが実証された.

結論:

  • ストレプトマイセスは 複雑なテルペノイドの持続的な生産のために 頑丈で未使用の微生物のシェーシとして機能します
  • 開発された代謝工学の戦略により,有価な酸化テルペノイド誘導体へのアクセスを可能にします.