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Updated: Sep 10, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

29.4K

パシフィック・バイオサイエンス,オックスフォード・ナノポア・テクノロジーズ,イルミナによる16S rRNAベースの土壌微生物群のプロファイルの比較評価

Vladimir Veselovsky1, Mikhail Romanov1, Polina Zoruk1

  • 1Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.

Frontiers in microbiology
|August 22, 2025
PubMed
まとめ

オックスフォード・ナノポア・テクノロジーズ (ONT) やパシフィック・バイオサイエンス (PacBio) のような長年のシーケンシング技術は,土壌の微生物を正確にプロファイルします. 微生物コミュニティの分析は,V4地域を除いて,シーケンシングプラットフォームまたはターゲット地域に関係なく,サンプルを効果的にクラスタ化します.

キーワード:
イルミナオックスフォード・ナノポーV3V4地域 パックバイオ環境サンプル全長 16S rRNA 配列化シーケンシングプラットフォームの比較土壌の微生物

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