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Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Updated: Sep 10, 2025

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Na&#239;ve-like State with Improved Multilineage Differentiation Potency
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RSeTヒト胚性幹細胞におけるナイブと形成性多能性変換に対する耐性

Kevin G Chen1, Kory R Johnson2, Kyeyoon Park1

  • 1NIH Stem Cell Unit.

Stem cells (Dayton, Ohio)
|August 22, 2025
PubMed
まとめ

RSeTヒト胚性幹細胞 (hESC) は,ナイブ状態とプライム状態と異なる独特の多能状態を表しています. これらの細胞は 独特の成長特性を示し ナイーブな多能性マーカーを欠いており 幹細胞状態の移行に関する新しい洞察を 提供しています

キーワード:
RSeTメディア細胞増殖人間の胚性幹細胞素朴な多能性についてトランスクリプトーム

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科学分野:

  • 幹細胞生物学
  • 発達生物学
  • ゲノミクス

背景:

  • 人間の胚性幹細胞 (hESC) はナイブ状態とプライムされた多能状態を示す.
  • 以前の研究では,様々なプロトコルから異質なナイヴ・プルリポテンツ状態を特定しました.
  • 多能状態の異質性を理解することは,幹細胞の応用において極めて重要です.

研究 の 目的:

  • 商業的な RSeT ベースの多能状態を特徴づける.
  • 異なる成長条件下でRSeT hESCの行動を調査する.
  • 他の多能状態との関係でRSeT hESCのトランスクリプトミカルおよび生化学的性質を定義する.

主な方法:

  • 細胞培養はノーマキシアとヒポキシアで
  • シングル・セル・プレッティング・エフェシエンス・アッセイ
  • 統合型トランスクリプトーム分析
  • 表面マーカー表現の分析 (SUSD2,CD75)
  • 信号経路依存性 (FGF2,JAK,TGFβ) に対する生化学的測定

主要な成果:

  • RSeT hESCは低酸素で成長できるが,成長とプレッティング効率は変動する.
  • RSeT hPSCには,ナイブおよび形成性多能性のトランスクリプトミックの特徴がない.
  • RSeT hESCは,初期の移植後の胚に似ており,プライマリ hESCに似ています.
  • 素朴な表面マーカーSUSD2とCD75は有意に表現されていません.
  • RSeT hESCは,FGF2に対する細胞系特異的依存性と,JAK/TGFβシグナル伝達に対する共依存性を示す.

結論:

  • RSeT hESCは,ナイヴな多能性の下流にある新しい多能状態を表しています.
  • RSeT媒体は,FGF2の活動を維持することによって,ナイヴの多能性を制限する可能性があります.
  • この研究は,in vitroの多能性状態の移行の理解を深める.