シンビオティックとコレンのインオ刺激が3世代に渡って性器組織に及ぼす世代間効果
Mariam Ibrahim1,2, Ewa Grochowska3, Marek Bednarczyk4
1Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland. miriam.ibrahim@pbs.edu.pl.
Scientific reports
|August 22, 2025
まとめ
産前エピジェネティック介入は,シナビオティックとコレンの作用により,鶏の世代間における遺伝子発現とDNAメチル化が変化することがあります. 繰り返された投与は,生殖組織に累積的な効果を示した.
科学分野:
- エピジェネティクス
- 発達生物学
- ゲノミクス
背景:
- エピジェネティックメカニズムは,遺伝的プロセスと現象的結果にとって極めて重要です.
- 産前曝露の世代を超えた影響はますます認識されています.
- 緑足ののようなニワトリは 生殖の表遺伝学の研究のモデルとして使われています
研究 の 目的:
- 遺伝子発現とDNAメチル化に対するインオブに注入された生物活性物質の世代間影響を調査する.
- 男性の性器に作用するシナビオティックとコレンの影響を 世代ごとに評価する
- 長期の生物学的効果に対する 産前エピジェネティック介入の可能性を調査する.
主な方法:
- 鶏の胚にSynbiotic PoultryStar® (PS) とコリンを注入する.
- F1,F2,F3世代にわたる対照,共生,共生コロイン群の確立.
- F2およびF3の雄性腺組織のトランスクリプトミカルおよび減少表現ビスルフィット配列化 (RRBS)
主要な成果:
- 遺伝子発現に対する世代間の影響は最小限であった.
- F1胚 (SYNCHs) にシンビオティックとコリンを併用すると,F3における有意な異なった発現遺伝子 (DEGs) と異なったメチル化領域 (DMRs) が誘発された.
- 重複投与 (SYNCHr) は,DEGとDMRの累積的な増加をもたらし,細胞骨組織,TGFβ,Wntシグナル伝達,および焦点結合などの経路に影響を与えました.
結論:
- 産前エピジェネティック介入は,生殖組織における遺伝子発現とDNAメチル化に永続的な変化を誘導することができます.
- エピジェネティック・モディフィケーションの程度は,投与戦略 (単発 vs 繰り返し) に依存する.
- 遺伝子発現とDNAメチル化における調整された変化は,世代を超えて標的を定めた表遺伝的改変の可能性を強調しています.
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