ペリラ精油:リステリア・モノサイトゲネスに対する天然の抗菌剤
Hai-Run Fan1, Man Hao2, Jie Yang1
1School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Journal of the science of food and agriculture
|August 23, 2025
まとめ
ペリラ エッセンシャルオイル (PEO) は,食物媒介の病原体であるリステリア・モノサイトゲネス (Listeria monocytogenes) と効果的に闘っています. この天然の代替品は 細菌の細胞構造を破壊し バイオフィルム形成を減少させ 食品の安全性の有望な解決策となります
科学分野:
- 食品科学
- 微生物学
- 自然製品 化学
背景:
- Listeria monocytogenesによって引き起こされる食品感染症の発生率が増加しています.
- 健康上のリスクのため,化学薬品の代替天然抗菌剤が必要である.
- L. monocytogenesに対するPerillaエッセンシャルオイル (PEO) の有効性に関する研究は限られている.
研究 の 目的:
- Listeria monocytogenesに対するペリラ精油 (PEO) の抗菌性を調査する.
- 食品安全における天然の抗菌剤としてのPEOの可能性を評価する.
主な方法:
- 乾燥したペリラの葉からPEOを抽出する.
- ガス染色体質量測定法 (GC-MS) を用いたPEO化合物の特定
- MIC/MBCの測定,SEMイメージング,表面水害性測定,バイオフィルムバイオマス定量測定,核酸/タンパク質漏れ測定,ゲル電泳,呼吸器鎖脱水酵素活性測定による抗菌性の評価
主要な成果:
- PEOは,MIC/ MBC値が1/4mgmL1であったと,L. monocytogenesに対する有意な抗菌活性を示した.
- SEMはPEO誘発の細菌細胞の変形と破裂を明らかにした.
- PEOはバクテリアの表面の疎水性を増加させ,バイオフィルム形成を減少させ,細胞壁/膜を破壊し,細胞内部の漏れを引き起こした.
- PEO治療は細菌のタンパク質含有量と呼吸鎖脱水素酵素の活性を著しく低下させた.
結論:
- ペリラ精油は,リステリア・モノサイトゲネスに対する強力な抗菌効果を発揮する.
- PEOは,食品におけるL.monocytogenesの汚染を制御する天然の抗菌剤として有望である.
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