中国サイクリン (Cyclina sinensis) の性差別と性ステロイドホルモンへの反応に関連する遺伝子の分子発現
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PubMedで要約を見る
まとめ
この要約は機械生成です。ホルモン治療は,幼生サイクリンシネシスの性決定を妨害し,性比とVitellogenin (Vg) やFoxl2のような重要な性差別遺伝子の発現を変化させた.
科学分野
- 海洋生物学
- 内分泌学
- 遺伝学
背景
- 脊椎動物における性差別を理解することは,水産養殖と生態学的研究にとって極めて重要です.
- ホルモンの調節は多くの水生生物の性発達に重要な役割を果たします.
- 商業的に重要なクラゲであるサイクリン・シネンシスは 性別決定のメカニズムをさらに調査する必要があります.
研究 の 目的
- シクリナ・シネシス (Cyclina sinensis) の性分化に関連する遺伝子の組織特異的発現と細胞下局部化を調査する.
- エストラディオールとテストステロンの治療による性比,性腺体指数,性肝体指数,ホルモン含有量,および若年C. sinensisの遺伝子発現の影響を分析する.
主な方法
- エストラディオール (E - 1, E - 50) とテストステロン (T - 1, T - 50) の濃度が異なるホルモン療法が,若年C. sinensisに施された.
- Vitellogenin (Vg),Tssk1,Dmrt1,Wnt4,Foxl2およびSox9を含む遺伝子のインサイトハイブリデーションを使用して遺伝子発現分析を行った.
- 性比,性腺体指数,肝臓体指数,内生性ホルモンレベルを測定した.
主要な成果
- 性別差異遺伝子 (Vg,Tssk1,Dmrt1,Wnt4) は成人性腺において有意に発現した.
- ホルモン治療は性比に有意な影響を及ぼさなかったが,遺伝子発現パターンに影響を与えた.
- エストラディオルは卵巣のVgを調節し,テストステロンは丸のTssk1発現と卵巣のFoxl2に影響した.
結論
- ホルモンの影響により,若いC. sinensisの正常な性決定と性差別遺伝子の発現が妨げられました.
- この研究は,C. sinensisの性発育が外来ホルモンに敏感であることを強調している.
- 発見は,この種の性決定と差別化の分子機構の洞察を提供します.
関連する概念動画
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
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The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...