関連する実験動画

Updated: Sep 10, 2025

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

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核融合微生物のトランスグルタミンゼ-タンパク質G変異体による本来のIgG Fab断片の近接性ベースのサイト固有のラベル付け

Koki Murozono1, Riko Nishioka1, Yoshirou Kawaguchi1

  • 1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.

New biotechnology
|August 23, 2025
PubMed
まとめ

新しいタグフリーメソッドは,エンジニアリングされた微生物トランスグルタミナーゼを使用して,抗体断片抗原結合 (Fab) のサイト固有のラベル付けを可能にします. このアプローチは,標的型がん治療と診断のための均質なファブコンジュガートを効率的に生成します.

キーワード:
抗体断片抗体結合タンパク質抗体と薬の結合体クロスリンク酵素による改変微生物のトランスグルタミンゼ

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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