セプシス関連ARDSにおけるエンドプラズマ網膜ストレスハブ遺伝子の特定と分析
Ling Gao1, Tingting Liu1, Xiaoyan Li2
1Department of Respiratory and Critical Care Medicine, Third Hospital of Shanxi Medical University Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, 030032, Shanxi Province, China.
Scientific reports
|August 23, 2025
まとめ
この研究では,STAT3とYWHAQがセプシスに関連した急性呼吸器不全症候群 (ARDS) と内プラズマ網膜ストレス (ERS) に関する重要な遺伝子として特定されました. これらの遺伝子は この重大な状態の 診断マーカーや治療標的として 潜在性を示しています
科学分野:
- ゲノミクス
- 分子生物学
- バイオ情報学
背景:
- セプシス誘発の急性呼吸器障害症候群 (ARDS) は重篤な合併症である.
- エンドプラズマ網膜ストレス (ERS) はセプシスに関連したARDSの病理生理学に関与しています.
研究 の 目的:
- セプシスに関連したARDSでERSに関連するハブ遺伝子を特定し,分析する.
- セプシスに関連したARDSの潜在的な診断マーカーと治療標的を評価する.
主な方法:
- GSE32707のデータセットで生物情報学と機械学習を活用した.
- 統合された差異遺伝子発現分析,WGCNA,機械学習アルゴリズム (LASSO,RF,SVM).
- RT- qPCRを用いた臨床サンプルでの検証結果
主要な成果:
- 438の差異的に発現する遺伝子と5つのハブ遺伝子:STAT3,HSPB1,YWHAQ,LCN2,SGK1を特定した.
- STAT3とYWHAQは臨床サンプルで有意な診断値と差異表現を示した.
- STAT3は上昇調節され,YWHAQは敗血症関連ARDSでは低下調節された.
結論:
- STAT3とYWHAQは,セプシスに関連したARDSにおける重要なERS関連遺伝子です.
- これらの遺伝子は 診断用バイオマーカーとして有望です
- STAT3とYWHAQは,セプシスに関連したARDSの潜在的治療目標です.
関連する概念動画
01:17Role of ER in the Secretory Pathway
5.6K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.6K
01:31Regulation of the Unfolded Protein Response
2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
01:37The Unfolded Protein Response
5.1K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.1K
01:30Other Stress Responses in Bacteria
65
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
65
01:32Export of Misfolded Proteins out of the ER
3.9K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.9K