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関連する概念動画

Protein Modifications in the RER01:26

Protein Modifications in the RER

5.6K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.6K
Conserved Binding Sites01:49

Conserved Binding Sites

4.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.3K
Protein-protein Interfaces02:04

Protein-protein Interfaces

13.2K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.2K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

11.3K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
11.3K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

7.1K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.1K

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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

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タンパク質の翻訳後の改変部位を予測するための効率的な機械学習フレームワーク

Heba M Elreify1, Fathi E Abd El-Samie2,3, Moawad I Dessouky4

  • 1Department of Electronics and Electrical Communications Engineering, Faculty of Electronic Engineering, Menoufia University, Menouf 32952, Egypt. hebamohamedibrahim09@gmail.com.

Scientific reports
|August 25, 2025
PubMed
まとめ
この要約は機械生成です。

HyLightKhibは,新しい計算フレームワークを使用して,リジン2-ヒドロキシイソブチリレーション (Khib) サイトを正確に予測します. この進歩は,タンパク質の調節を理解し,標的治療を開発するのに役立ちます.

キーワード:
ESMについてライトGBMリジン2ヒドロキシイソブチリル化機械学習相互の情報提供翻訳後の修正タンパク質の言語モデル

さらに関連する動画

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

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A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

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関連する実験動画

Last Updated: Sep 10, 2025

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

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A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

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科学分野:

  • 生化学と分子生物学
  • コンピュータ生物学
  • プロテオミクス

背景:

  • 翻訳後の改変 (PTM) はタンパク質の機能を調節し,リジン2-ヒドロキシイソブチリル化 (Khib) は重要な細胞プロセスに関与する.
  • 実験的なKhibサイトの識別は,資源集約的な方法と一時的な変更の性質のために困難です.

研究 の 目的:

  • Khib サイトを予測するための正確で効率的な計算ツールを開発する.
  • 現在の実験的なKhib識別技術の限界を克服するために.

主な方法:

  • Light Gradient Boosting Machineを利用した計算フレームワークであるHyLightKhibを導入した.
  • 進化的スケールモデリング (ESM-2) の埋め込み,構成,移行,分布 (CTD) の記述,およびアミノ酸の物理化学的性質を組み合わせたハイブリッド特性抽出戦略を採用した.
  • 相互情報を利用した機能選択を最適化.

主要な成果:

  • HyLightKhibは,それぞれ0. 893,0. 876,0. 847のAUC- ROCスコアで,多様な生物 (人間,寄生虫,米) に関する高い予測性能を達成しました.
  • DeepKhibやResNetKhibのような 既存の予測を上回りました
  • XGBoostとCatBoostの実装と比較して優れたパフォーマンスを示した.

結論:

  • HyLightKhibはKhibのサイト予測のための高度なコンピューティングアプローチを提供しています.
  • 機能性プロテオミクスのための予測精度と貴重な生物学的な洞察を提供します.
  • PTMを標的とする治療法の開発に直接的な影響がある.