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関連する概念動画

MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.0K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Updated: Sep 10, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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ERHは,クラスター支援を超えてプライマリマイクロRNA処理を促進します.

Harim Jang1,2, Junyoung Park1,2, V Narry Kim3,4

  • 1Center for RNA Research, Institute for Basic Science, Seoul, Korea.

Nature communications
|August 25, 2025
PubMed
まとめ
この要約は機械生成です。

ERHとSAFB2は,プライマリマイクロRNA (プリ-ミRNA) を処理するのに役立つ補助因子です. この研究は,ERHがプリミRNAの処理を大きく強化し,SAFB2がクラスタ化されたプリミRNAを特に支援することを明らかにしています.

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関連する実験動画

Last Updated: Sep 10, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

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科学分野:

  • 分子生物学
  • RNA 生物学
  • 遺伝子規制

背景:

  • マイクロRNA (miRNA) の生殖は遺伝子調節に不可欠である.
  • マイクロプロセッサ複合体 (DROSHA-DGCR8) は,プライマリミRNA (プリミRNA) からミRNAの成熟を開始する.
  • ERHとSAFB2は,特にクラスター支援において,マイクロプロセッサの機能を強化する補助因子として知られています.

研究 の 目的:

  • プリミRNA処理におけるERHとSAFB2の異なる分子メカニズムを解明する.
  • pri-miRNAクラスター支援におけるその役割を理解する.
  • miRNA 成熟におけるそれらの機能のモデルを提案する.

主な方法:

  • プリミRNA処理アッセイを用いてERHとSAFB2の機能を調査した.
  • ERH と SAFB2 の単独およびクラスタ化された pri- miRNA への影響を分析した.
  • ERHとSAFB2の減少の影響を評価するために,ノックダウン実験を使用した.

主要な成果:

  • ERHは,ゲノムのコンテキスト (単独またはクラスタ化) にかかわらず,プリミRNA処理を大きく強化します.
  • ERHのノックダウンは,効率的なものと比べると,非最適の pri- miRNAヘアピンに不釣り合いな影響を及ぼします.
  • SAFB2は,特にクラスター内のサブ最適のプリミRNAヘアピン処理を容易にする.

結論:

  • ERHとSAFB2は,プリミRNAのクラスター支援において異なる役割を演じている.
  • 新しいモデルでは,SAFB2がヘアピン間のマイクロプロセッサの転送を容易にする.
  • ERHは,非最適のプリミRNAの効率的な処理を可能にすることを提案しています.