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Updated: Sep 10, 2025

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

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単細胞誘導結合プラズマ質譜を用いた細胞におけるNIS依存性ヨウ素蓄積の非放射量測定

Tanya Mehra1, Aimee E P Mcloughlin2, Nikolay Solovyev3

  • 1School of Pharmacy, School of Health Sciences, College of Medicine and Health, University of Birmingham, B15 2TT, UK; Department of Life Sciences, ATU Sligo, Atlantic Technological University, F91 YW50, Sligo, Ireland.

Talanta
|August 26, 2025
PubMed
まとめ

この研究では,個々の細胞内のヨウ素を定量化するための単細胞ICP-MSを導入し,ヨウ素代謝に関する新しい洞察を提供します. この方法では 甲状腺細胞におけるヨウ素の蓄積パターンが明らかになり 伝統的な技術の限界を克服しました

キーワード:
ヨウ素NIS について唾液腺シングル・セル・インダクティブ・カップリング・プラズマ・マススペクトロメトリー (scICP-MS)ヨド酸ナトリウム甲状腺

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
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