帽子から首輪まで:Neurospora crassaの内細胞首輪のオントジェニー

Marisela Garduño-Rosales^1,2, Caleb Oliver Bedsole³, Brian D Shaw³

¹Departamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada 22860, B.C., Mexico.

Journal of fungi (Basel, Switzerland)

|August 27, 2025

まとめ

菌類の内細胞は,ヒファが成長するにつれて亜ヒファの首輪に移動するアピカルキャップを形成する. このコラーは偏った成長を維持するために重要なものですが初期の確立には欠かせません

科学分野:

細胞生物学
菌類学
キノコの発達

背景:

糸状菌の内細胞化は,内細胞コラーと呼ばれるサブアピカル領域に限定される.
膜のリサイクルと分極化した成長を維持するために不可欠です.

研究の目的:

*ニューロスポラ・クラサ*の内細胞カラーの発達起源 (オントジェニー) を調査する.
ハイファの成長過程における内細胞と外細胞の空間的および時間的関係を理解する.

主な方法:

コンフォカル顕微鏡を用いてフィムブリンで標識された内細胞パッチを追跡した.
コニディアの発芽,ヒファの分岐,損傷後の再生を観察した.

主要な成果:

内細胞のパッチは最初はヒファの先端に蓄積され,アピカルキャップを形成した.
アピカルキャップはサブアピカルカラーに再編成され,延長率とスピッツェンケイパーの出現と相関する.
クリティカルな伸び率を超えた後にカラー形成が観察され,エクソサイトーシスがエンドサイトーシスを置換することを示唆した.

結論:

エンドサイトカラーは, *Neurospora crassa* の極化成長を維持するために不可欠ですが,確立することはできません.
エクソサイトーシスは,ヒファの頂点からエンドサイトーシスを移動させ,カラー形成につながります.
発見は,真菌のカラー形成と内細胞と外細胞のプロセスの調整の統一モデルに寄与する.

キーワード:

ニューロスポラ・クラッサエンドサイトカップエンドサイトーシスエクソサイトーシスサバピカル・エンドサイトカラー

さらに関連する動画

17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

14.6K

10:02

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

Published on: October 23, 2016

11.0K

関連する概念動画

01:32

Pinching-off of Coated Vesicles

3.2K

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

3.2K

01:12

Clathrin Coated Vesicles

7.2K

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

7.2K

01:28

Maturation of Endosomes

4.4K

The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...

4.4K

01:45

Vesicular Tubular Clusters

2.6K

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

2.6K

01:45

ER Retrieval Pathway

3.9K

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...

3.9K

01:33

Coat Assembly and GTPases

3.6K

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...