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Updated: Jun 15, 2026

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
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培養されたヒト細胞のSBEMベースの超構造分析のための最適化されたプロトコル

Natalia Diak1, Łukasz Chajec2, Agnieszka Fus-Kujawa1

  • 1Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752 Katowice, Poland.

Methods and protocols
|August 27, 2025
PubMed
まとめ

連続ブロック面スキャニング電子顕微鏡 (SBEM) を 人間の細胞に最適化しました この新しいプロトコルは,幹細胞と線維細胞の超構造分析を強化し,より明確な安全性を提供します.

キーワード:
SBEMプロトコルフィブロブラストiPSC について細胞の超構造

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.