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Reporter Genes02:11

Reporter Genes

11.8K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

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Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
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Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

148
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
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Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

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Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

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遺伝子発現のプログラム可能なタイマー・スイッチ

Cheng Huang1, Jie Zhao1, Zichun Tan1

  • 1Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou, China.

Science advances
|August 27, 2025
PubMed
まとめ
この要約は機械生成です。

プログラム可能な遺伝子発現タイマー スイッチで 反応時間は数時間から数日間です この合成生物学のツールは 代謝工学や生物医学での応用のために 精密に遺伝子活動を制御します

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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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科学分野:

  • 合成生物学
  • 遺伝子工学
  • 分子生物学

背景:

  • 既存の遺伝子発現タイミングシステムは,複雑なレギュレータの相互作用により,一時的な調節性が制限されています.
  • 遺伝子発現のタイミングを正確に制御することは,様々なバイオテクノロジーの応用において極めて重要です.

研究 の 目的:

  • プログラム可能な新型遺伝子発現タイマー スイッチを開発する
  • 代謝経路と生物学的プロセスを制御する このタイマーの有用性を示すために

主な方法:

  • アデニン塩基エディター (ABE) による DNA カセット編集を利用した合成遺伝子回路を設計した.
  • DNAカセットの数を調整することで プログラムされた反応時間
  • E. coli のカロテノイド生物合成遺伝子とバクテリアの自己分解システムを制御するために BioFuse を適用した.

主要な成果:

  • BioFuseはプログラム可能な遺伝子発現のタイミングを数時間から数日間まで可能にします
  • コリ菌のライコペンの生成を制御し 増殖から切り離し 収穫量を増やします
  • バクテリアの自己分解システムで 適時かつ効率的なタンパク質の放出を促進する.

結論:

  • BioFuseは細菌の遺伝子発現と代謝活動の精密で広範囲の時間的制御のための汎用的なツールを提供します.
  • この技術は,産業用バイオテクノロジーとバイオメディシンに潜在的応用があります.