乳酸バクテリアのバイオフィルムを合成し,シロージの発酵を調節するための培養基を準備するために,米のストローを使用する
Zhumei Du1, Jiachen Fang2, Huili Pang3
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Bioresource technology
|August 27, 2025
まとめ
この研究により,低コストのライスストロー媒介が作られ,優れたシロージ発酵のための乳酸細菌 (LAB) バイオフィルムが作られました. 開発されたLABバイオフィルムは,シロージの質を向上させ,有益な微生物のシフトを促進しました.
科学分野:
- 農業科学
- 微生物学
- バイオテクノロジー
背景:
- 米のような作物の副産物は 十分に活用されていない資源です
- シラージの発酵の質は,家畜の飼料の保存に不可欠です.
- 有害な微生物がシロージを支配し,その質を損なう.
研究 の 目的:
- 乳酸バクテリア (LAB) のバイオフィルム生産のために米のストローを用いた低コストで自然な培養媒体を開発する.
- LABバイオフィルムがシロージの発酵の質を改善する効果を調査する.
- シリアス発酵中のLABバイオフィルムの微生物コミュニティ構造への影響を分析する.
主な方法:
- 自然培養基を作るのに米のストローを使用した.
- 乳酸細菌 (LAB) 株FG1 (Lactiplantibacillus plantarum) とTH14 (Lacticaseibacillus casei) を利用した.
- 培養媒介とLAB株を使って,果樹園の草とティモシー草からシラージを製造した.
- 発酵品質のパラメータ (pH,アンモニア窒素,乳酸) を測定した.
- 微生物のコミュニティ構造と代謝経路を分析した.
主要な成果:
- FG1媒体を用いて発酵したシラージは優れた品質を示した (pH<3.6,アンモニア窒素<0.7g/kg FW,乳酸>1.1g/kg FW).
- LABバイオフィルムは,グラム陰性からグラム陽性細菌へのシフトを促進し,Lactiplantibacillus plantarum (35. 4%) が優勢でした.
- バイオフィルムは微生物の共生ネットワークを調節し,炭水化物の代謝を促進し,アミノ酸経路を阻害しました.
結論:
- 低コストのライスストローベースの媒体は,高品質のシロージのためのLABバイオフィルムを効果的に生産しました.
- 設計されたLABバイオフィルム構造は,シロージの発酵と微生物の動態を大幅に改善しました.
- このアプローチは低コストで高品質なシロージの生産に 持続可能で効果的な方法を提供する.
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