関連する実験動画

Updated: Sep 10, 2025

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

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タンパク質言語モデルの支援によるサイクロデキストリナーゼの誘導進化 精度のα-O-オリゴサカリド合成を可能にする

Ting Nie1, Zhenxin Yan2, Hao Liu3

  • 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Institute of Key Biological Raw Material, Shanghai Academy of Experimental Medicine, Shanghai 201401, China.

Bioresource technology
|August 27, 2025
PubMed
まとめ

研究者は精密なアルファ-O-オリゴサカリド合成のためにサイクロデキストリナーゼ酵素を設計しました. この新しいグリコシドヒドローラゼシステムは,現在のグリコバイオロジーの方法の限界を克服して,収穫量と特異性を大幅に改善します.

キーワード:
サイクロデクストリナーゼ一般的なタンパク質言語モデルモチーフプローブ多目的の最適化α-O-オリゴサカリド

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関連する概念動画

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
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