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Updated: Apr 7, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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3T3-L1細胞における家事遺伝子RT-qPCRデータを検証するための計算方法の比較評価

Zhenya Ivanova1, Natalia Grigorova1, Valeria Petrova1

  • 1Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.

Biomedicines
|August 28, 2025
PubMed
まとめ

この研究では,ポストバイオティクスの治療を受けた脂肪細胞における正確な遺伝子発現分析のための最も安定した管理遺伝子を特定した. HPRTは,RT-qPCRの正常化のための最も信頼性の高い内部コントロールであることが判明しました.

キーワード:
3T3-L1 についてHPRT についてL. パラケーシーRT-qPCR について標準化参照遺伝子

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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