関連する実験動画

Updated: Sep 9, 2025

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

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[ファグ免疫降水配列解析技術の進歩]

Yuhao Zhu1,2,3,4, Wenlong Zhu1,2,3,4, Yujie Lai1,2,3,4

  • 1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|August 28, 2025
PubMed
まとめ

菌糸体免疫降水配列解析 (PhIP-Seq) は,タンパク質とペプチドの結合を分析するための費用対効果の高い方法である. この高通量技術は 何百ものタンパク質のペプチド標的を検出し 病気の検出やバイオマーカーの発見に役立ちます

キーワード:
診断技術ペプチドライブラリファグ免疫降水配列化 (PhIP-Seq)ワクチン開発

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関連する概念動画

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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