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関連する概念動画

Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
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The Eukaryotic Promoter Region02:40

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Gene Duplication and Divergence02:37

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The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
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Gene Conversion02:08

Gene Conversion

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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ディープ・ジェネラティブ・アンド・ダイナミック・エボリューション・アルゴリズムに基づくデ・ノボ・プロモーター設計方法

Yijun Gu1, Jianye Su2, Junfeng Xia2

  • 1Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, 111 Jiulong Road, Hefei 230601 Anhui, China.

Nucleic acids research
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まとめ
この要約は機械生成です。

調整可能な遺伝子発現レベルを持つ 合成プロモーターを設計するための 新しい方法である PromoDGDEを開発しました このアプローチは,E. coliとS. cerevisiaeの機能的プロモーターを作成することによって,代謝工学と遺伝子治療を強化します.

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科学分野:

  • 合成生物学
  • 計算生物学
  • 分子生物学

背景:

  • プロモーターは遺伝子発現の制御に不可欠であり,代謝工学と遺伝子治療に不可欠です.
  • 現在のプロモーター設計方法は,しばしば高い表現を優先し,調整可能な規制強度の必要性を無視します.

研究 の 目的:

  • 特定の表現レベルを持つ合成プロモーターを設計するための新しい深層学習フレームワークであるPromoDGDEを導入する.
  • この方法がEscherichia coliとSaccharomyces cerevisiaeに有効であることを実証する.

主な方法:

  • 自然プロモーター配列の特徴を学習し,新しい候補を生成するためにDiffusion-GANを使用します.
  • 合成プロモーター配列のダイナミック最適化のための強化学習と進化アルゴリズムを使用する.

主要な成果:

  • PromoDGDEはシリコンで既存の方法を上回り,望ましい機能を持つ生物学的に重要なプロモーターを生成しました.
  • 合成プロモーターの発現活性が確認され,予想される規制効果が60%以上達成された.

結論:

  • PromoDGDEは 合成プロモーターの設計に 有効で柔軟なソリューションを提供しており 厳格な規制規制が適用されます
  • この方法は,代謝経路の構築と遺伝子治療を含む合成生物学におけるアプリケーションの進歩に重要な意味を持っています.