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Updated: Sep 9, 2025

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マススペクトロメトリー (NESSI-MS) を用いた原生および内生SUMOサイト識別

Caroline L E Lennartsson1, Ivo A Hendriks1

  • 1Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Methods in molecular biology (Clifton, N.J.)
|August 28, 2025
PubMed
まとめ

この研究では,ネイティブの小型ウビキチン型変異体 (SUMO) サイトを特定するための新しいプロテオミクス法であるNESSI-MSを導入しています. このテクニックは,遺伝子組み換えなしに生物系における内生性SUMOylationの正確なマッピングを可能にします.

キーワード:
データに依存した取得内生性ラベルなしの定量化マススペクトロメトリーPTMプロテオミクスSUMO についてSUMOYlatedライシンマッピングSUMOylation 溶解について

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