関連する実験動画

Updated: May 7, 2026

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination
16:02

Quantitative FRET Förster Resonance Energy Transfer Analysis for SENP1 Protease Kinetics Determination

Published on: February 21, 2013

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SENP/ULPファミリーのSUMOイソフォーム偏好を比較するためのフローロメトリックアッセイ

Nathalia Varejão1, Lucía Sánchez-Alba2, David Reverter3

  • 1Institut de Biotecnologia i de Biomedicina (IBB) and Dept. de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. nathalia.varejao@uab.cat.

Methods in molecular biology (Clifton, N.J.)
|August 28, 2025
PubMed
まとめ

この研究は,SENP/ULP酵素の基板特異性を比較する競争試験を導入する. この方法は,酵素活性を理解するのに役立つ,様々なユビキチンのような変形剤 (UbL) に対する酵素親和性を決定するのに役立ちます.

キーワード:
DUB についてデコンジュガーションIC50 についてイソペプチダゼSENPSUMO についてULP についてウビキチン

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