関連する実験動画

Updated: Sep 9, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

関連から因果関係へ:COVID-19の病原性における長い非コーディングRNAの役割を解明する

Tianfei Yu^1,2, Yunhan Zhang^3,4, Haolan Zhang^3,4

¹Department of Biotechnology, College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, 161006, China. yutianfei2001@163.com.

Virology journal

|August 28, 2025

まとめ

3つの長いノンコーディングRNA (lncRNA) は,コロナウイルス病 2019 (COVID-19) の診断の可能性を示しています. これらの lncRNAs,H19,TUG1,およびCRNDEは,疾患に関連する炎症および線維症のバイオマーカーとして機能する可能性があります.

科学分野:

分子生物学
生物化学
ゲノミクス

背景:

長い非コーディングRNA (lncRNAs) は,疾患の病原性における役割としてますます認識されています.
コロナウイルス病 2019 (COVID-19) は,複雑な炎症性および線維性反応を伴う.
COVID-19の診断と予後のための信頼できるバイオマーカーを特定することは極めて重要です.

研究の目的:

COVID-19における3つの特定のlncRNA (H19,TUG1,CRNDE) の診断の可能性を調査する.
COVID-19に関連した炎症および線維症におけるこれらの lncRNA の生物学的重要性を調査する.
lncRNA発現に基づいてCOVID-19を予測する機械学習モデルの有用性を評価する.

主な方法:

COVID-19患者のH19,TUG1,CRNDE発現レベルの分析.
機械学習アルゴリズムの適用により,COVID-19の診断を予測するモデルを開発する.
lncRNA発現と炎症と線維症に関連する臨床パラメータの相関分析

主要な成果:

COVID-19患者では,H19,TUG1,CRNDEの有意な差異表現が観察されました.
機械学習モデルは,IncRNAプロファイルに基づいてCOVID-19症例を識別する可能性を実証した.
この研究は,COVID-19の基礎となる生物学的プロセスにおけるこれらのlncRNAの役割を示唆しています.

結論:

H19,TUG1,CRNDEはCOVID-19の潜在的な診断バイオマーカーです.
これらのlncRNAはCOVID-19の炎症性および線維性病理に寄与する可能性があります.
これらの発見を検証し,治療上の意味を探るため,さらなる研究が必要である.

キーワード:

COVID-19のバイオマーカー診断の可能性炎症と線維症長い非コーディングRNA (lncRNAs)機械学習アプリケーション

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関連する概念動画

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...