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Updated: Sep 9, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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自動化されたエドマン分解を用いた二硫化結合分析におけるDIPTH-システインとPTH-システイン

Toni Kühl1, Yomnah Y Elsayed2, Alexander Terekhov1

  • 1Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, Bonn, Germany.

Journal of peptide science : an official publication of the European Peptide Society
|August 29, 2025
PubMed
まとめ

この研究は,エドマンシーケンスを用いてペプチドにおける二硫化物ブリッジ分析を強化する. PTH誘導体を比較することで,研究者はシステインの結合性を正確に特定し,システインに富んだタンパク質の構造の解明を簡素化します.

キーワード:
エドマン劣化コノトキシンシステインディスルファイドブリッジインスリンペプチド

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