関連する実験動画

Updated: Sep 9, 2025

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

14.0K

(PP-InsP) 免疫洗浄と質量スペクトロメトリーによる標的の特徴化のための親和感探査

Jaime A Isern1, Abhirup Majumdar1,2, Annika Richter1,2

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.

Methods in molecular biology (Clifton, N.J.)
|August 29, 2025
PubMed
まとめ

研究者は,バイオチニル化プローブを使用して,細胞サンプルからイノシトール・フォスファート (InsPs) とパイロフォスファート (PP-InsPs) と相互作用するタンパク質を分離するために,より簡単な方法を開発しました.

キーワード:
アフィニティベースのタンパク質プロファイリング (AfBPP)バイオチニル化イノシトール・フォスファート・アフィニティ・プローブラベルなしの定量化マススペクトロメトリーウエスタン・ブロット

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関連する概念動画

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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