ゴールデン・オーブス - 発酵によるオーブスミルクの天然強化
Emmelie Joe Freudenberg Rasmussen1, Jesper Holck2, Peter Ruhdal Jensen1
1National Food Institute, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
FEMS microbiology letters
|August 29, 2025
まとめ
この研究では,ラクトコックス・ラクティス菌を用いて ビタミンB2 (リボフラビン) を含んだ植物性飲料を改良した. 酵素治療とアルギニンサプリメントは,オートミルクのリボフラビン生成を著しく増加させた.
科学分野:
- 食品科学
- 微生物学
- バイオテクノロジー
背景:
- 植物性飲料には 必須のビタミンBが欠けていて 強化が必要になります
- 乳酸バクテリア (LAB) デリバティブ,特にラクトコッカス・ラクティス (L. lactis) は,ビタミンB2 (リボフラビン) の生産のために設計することができます.
- L. ラクティスのリボフラビン生成は,基質の利用可能性と環境条件によって影響を受けます.
研究 の 目的:
- 遺伝子工学によるL. lactis菌株が,大豆やオートベースの飲料にリボフラビンを添加する可能性を評価する.
- これらの植物ベースのマトリックスでリボフラビン生産を最適化する方法を調査する.
- L. ラクティスによるリボフラビン生物合成を制限し強化する重要な要因を特定する.
主な方法:
- 飲料濃縮試験のために,リボフラビン産のL.ラクティス菌株 (ER10,ALE13,LDH13) を3種類選択した.
- 静的および空気状態のソーヤミルクとオートミルクで培ったL. lactis.
- プロテアゼ (Alcalase®,Flavourzyme®) で処理されたオートミルクは,タンパク質/アミノ酸の生物利用性を改善します.
- 酵素で処理されたオートミルクをアルジニンで補充し,リボフラビン産生への影響を評価した.
主要な成果:
- 静的条件下では,ALE13と比較して,株ER10は大豆ミルクでリボフラビンの産出量が高かった.
- アエレーションは一般的にリボフラビン産量を増加させ,特にLDH13株を増加させた.
- オートミルクの酵素処理は,リボフラビン生成をL. lactis (約 600%増加した.
- アルギニンの補給により,処理されたオートミルクのリボフラビン含有量はさらに約8mg/Lに増加しました.
結論:
- エンジニアリングされたL. lactis株は,植物性飲料のリボフラビン強化に有効です.
- 亜基質の生物利用可能性,特にアミノ酸含有量 (例えばアルジニン) を最適化することは,オートミルクにおけるリボフラビン生産を最大化するために不可欠です.
- 酵素前処理と制御されたエアレーションは,オートミルクのような挑戦的なマトリックスでのリボフラビン産量を高めるための重要な戦略です.
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